Testing for FNAIT, PTP and TRALI investigation

Our lab carries out human platelet antigen (HPA) and human neutrophil antigen (HNA) genotyping and antibody screening.

Fetal/neonatal alloimmune thrombocytopenia (FNAIT)

HPA antibody and genotype testing is used in suspected cases of FNAIT.

Our form for initial request of FNAIT investigation should be completed with SNBTS clinical staff.

If you require a copy of the FNAIT initial request form, please contact NSS.handi@nhs.scot

Post-transfusion purpura (PTP)

HPA antibody and genotype testing is used in suspected cases of PTP.

Transfusion-related acute lung injury (TRALI)

Human leucocyte antigen (HLA) and human neutrophil antigen (HNA) antibody screening are used in suspected cases of TRALI.

Our form for TRALI investigation should be completed with SNBTS clinical staff.

If you require a copy of the TRALI Investigation form, please contact NSS.handi@nhs.scot

You should note that all samples for HNA testing and confirmatory HPA antibody testing by monoclonal antibody-specific immobilization of platelet antigen (MAIPA) will be sent to the NHS Blood and Transplant (NHSBT) Platelet and Granulocyte Reference Lab in Filton, Bristol.

Sample requirements

All samples must be sent with the relevant request form and must be less than 48 hours old when received by the lab.

FNAIT              

• Mother: 1 x 5ml clotted sample and 1 x 5ml EDTA samples
• Father: 5ml EDTA sample
• Baby: 1ml EDTA sample

PTP

• Patient: 2 x 7ml clotted and 1 x 5ml EDTA samples

TRALI              

• Patient: 2 x 5ml EDTA samples

If you require a copy of the Test Request forms and Samples Acceptance Criteria, please contact NSS.handi@nhs.scot